Bio. Sci. 4 - Microbiology Laboratory Syllabus
Harriet Wilson, Instructor
The laboratory syllabus included here is provided in sections. This document (currently sold in the campus bookstore) has been converted into electronic files for your convenience. The laboratory syllabus is intended to provide students with electronic access to the information necessary for completing the laboratory portion of Sierra College microbiology classes (Bio. Sci. 4 and 8A-8B). Laboratory Syllabus - Copyright 2017 Harriet S. Wilson. All Rights Reserved.
Laboratory Syllabus Sections (PDF):
Laboratory Syllabus Cover - July 2017
Laboratory Procedures and Safety Regulations
Microscopy, Microscopic Measurement & Observation/scientific Illustration
Culture of Microorganisms and Media Preparation
Aseptic Technique, Streak Plates and Pure Culture Technique
Staining - Direct and Indirect Stains
Staining - Gram stain and KOH Test
Staining - Endospore, Capsule and Flagellar Stains
Introduction to Bacteria and Cyanobacteria
Introduction to Multicellular Parasites
Food Microbiology - Fermented and Cultured Foods
Study Guide for Laboratory Exam 1
Determination of Microbial Numbers
Use of Selective and Differential Media and Replica Plating
The Scientific Method and Microbial ID Supplements
Charbohydrate Metabolism, Catalase, and Oxidase Tests
Lysine Decarboxylation, Citrate Utilization, Coagulase and CAMP Test
Gelatin Liquefaction, TSI, SIM, Urea Hydrolysis and Nitrate Reduction
Application of the PCR in Bacterial Identification
Nucleotide Sequencing, Electropherogram Evaluation and Bioinformatics
Supplement for Laboratory Exercises 17 and 18
The Mini-screen, Rapid Isolation of Plasmid DNA
Gel Electrophoresis of DNA Samples
Restriction Endonuclease Digestion of DNA and RFLP
Competent Cell Preparation and Transformation
Introduction to Viruses, Phage Typing and Isolation of Coliphage
Bacteriophage Reproduction and Plaque Formation
Microbial Control (Effects of Temperature, UV Light, Disinfectants and Antiseptics)
Antimicrobial Suseptibility Testing
Bacteriological Examination of Water
Composition of Blood and White Cell Study
Diagnostic Immunology (Precipitation, Agglutination and ELISA)
Semester Project grading rubric
Study Guide for Laboratory Exam 2
Appendix A and B and References
Answers to Laboratory Exercise Questions
Bio. Sci. Recommended Study Techniques (Web Version)