Simulated RFLP Gels
Carefully Prepared for
Bio. Sci. 4 Microbiology Students,
Harriet Wilson's Classes
Fall 2016
RFLP 1 Gel #1 containing samples 1-5 in lanes as numbered. Lane #6 contains a 1Kb ladder (for size comparison). The fragment sizes in each lane are as follows:
Lane #1 = 602, 359, 209, 90, 81, 79, 71, 16
Lane #2 =471, 371, 207, 144, 121, 65, 42, 31
Lane #3 =572, 374, 207, 98, 86, 79, 48, 42
Lane #4 =396, 310, 286, 206, 166, 55, 50
Lane #5 =427, 210, 209, 206, 166, 144, 100, 42
Lane #6 = 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100
RFLP 2 Gel #2 containing samples 6-10 in lanes 1-5. Lane #6 contains a 1Kb ladder (for size comparison). The fragment sizes in each lane are as follows:
Lane #1 (sample #6) =475, 286, 255, 223, 206, 45
Lane #2 (sample #7) =638, 475, 186, 88, 61, 52, 26
Lane #3 (sample #8) =478, 324, 288, 206, 206
Lane #4 (sample #9) =470, 374, 208, 207, 165, 54, 32
Lane #5 (sample #10) =379, 374, 207, 172, 165, 98, 52, 42, 21
Lane #6 = 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100
RFLP 3 Gel #3 containing samples 11-15 in lanes 1-5. Lane #6 contains a 1Kb ladder (for size comparison). The fragment sizes in each lane are as follows:
Lane #1 (sample #11) = 615, 265, 209, 123, 88, 86, 81, 52
Lane #2 (sample #12) = 600, 451, 228, 144, 9
Lane #3 (sample #13) =475, 310, 286, 205, 165, 42, 6
Lane #4 (sample #14) = 291, 286, 265, 213, 209, 161, 45, 19
Lane #5 (sample #15) = 540, 466, 209, 128, 79, 42, 18, 13
Lane #6 = 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100
RFLP 4 Gel #4 containing samples 16-20 in lanes 1-5. Lane #6 contains a 1Kb ladder (for size comparison). The fragment sizes in each lane are as follows:
Lane #1 (sample #16) = 614, 472, 209, 173, 35
Lane #2 (sample #17) = 209, 202, 189, 179, 144, 120, 106, 81, 65, 44, 42, 42, 32
Lane #3 (sample #18) = 403, 211, 209, 207, 162, 162, 101, 46
Lane #4 (sample #19) = 350, 245, 223, 223, 202, 147, 58
Lane #5 (sample #20) = 310, 286, 235, 217, 209, 206, 8
Lane #6 = 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100
RFLP 5 Gel #5 containing samples 21 in lane #5. Lane #6 contains a 1Kb ladder (for size comparison). The fragment sizes in each lane are as follows:
Lane #1 (sample #21) = 481, 468, 329, 204, 35
Lane #2 (sample #22) = 615, 214, 209, 174, 127, 83, 75, 51
Lane #3 (sample #23) = 404, 376, 207, 169, 165, 98, 51, 42
Lane #4 (sample #24) = 392, 280, 226, 207, 195, 180, 42
Lane #5 (no sample)
Lane #6 = 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100
Copyright 2012 Sierra College Biological Sciences Department. All Rights Reserved.
